รศ.ดร.ปาริชาติ พุ่มขจร
Assoc. Prof. Dr.Parichat Phumkhachorn
Department : ภาควิชาวิทยาศาสตร์ชีวภาพRoom : CBL2207
Phone : 4212
Mobile : 4212
E-mail : scpariph@gmail.com
Education
Khon Kaen University (Thailand) Medical Technology (B.Sc.)
Mahidol University (Thailand) Microbiology (M.Sc.)
Khon Kaen University (Thailand) Biology (Ph.D.)
Research interest
Dr. Parichat Phumkhachorn’s main research of interest is in the field of Medical Microbiology and Immunology. Her present research focuses on finding bacteriophages for use as prophylactic and therapeutic agents against pathogenic bacteria causing animal and human diseases.
Research grant
1990: University Development Commission (UDC) scholarship for master degree
2006: University Development Commission (UDC) scholarship for Ph.D. degree
Working experience
Program Committee: 2018-Present
Bachelor of Science (B.Sc.) Microbiology Program
Course Coordinator: 1994-Present
Bachelor of Science (B.Sc.) Microbiology Program
Fundamentals of Microbiology Laboratory (Compulsory course)
Immunology (Compulsory course)
Pathogenic Microbiology (Elective course)
Director/Co-director of research funding2000: National Research Council of Thailand (NRCT); 296,500 Baht
Screening of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Fermented Foods.
2001: National Research Council of Thailand (NRCT); 210,000 Baht
Study of Cultural Conditions for the Growth and Bacteriocin Production of Lactococcus sp. Isolated from Fermented Food.2001: National Research Council of Thailand (NRCT); 287,000 Baht
Screening of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Meat.
2002: National Research Council of Thailand (NRCT); 514,000 Baht
Studies on Molecular Properties of a Bacteriocin Produced by Lactobacillus lactis PS21.
2008: National Research Council of Thailand (NRCT); 427,400 Baht
Development of a Transformation Method Using Glass Bead for Introducing DNA into Lactic Acid Bacteria.2009-2010: Thailand Research Funds (TRF); 480,000 Baht
Detection and characterization of a bacteriophage against nisin producing-Lactococcus lactis subsp. lactis TFF221, a potential starter culture in Thai fermented foods2010-2011: National Research Council of Thailand (NRCT); 424,600 Baht
Isolation of bacteriophage specific to a giant tiger prawn pathogen, Vibrio harveyi, as a candidate for disease control2011: National Research Council of Thailand (NRCT); 343,200 Baht
Isolation and characterization of lytic phage against Lactococcus lactis RP359, Kem-Buk-Nud starter culture
2012 (continued): National Research Council of Thailand (NRCT); 348,700 Baht
Isolation and characterization of lytic phage against Lactococcus lactis RP359, Kem-Buk-Nud starter culture2013-2014: National Research Council of Thailand (NRCT); 697,400 Baht
Isolation of bacteriophage specific to a fish pathogen, Streptococcus agalactiae, for use as a disease biocontrol agent in tilapia
2015: National Research Council of Thailand (NRCT); 299,500 Baht
Antimicrobial ability of bacteriophage against drug resistant Escherichia coli producing extended-spectrum B-lactamase (ESBL)
2017: National Research Council of Thailand (NRCT); 264,000 Baht
Isolation and Characterization of Bacteriophage specific to a Fish Pathogen, Edwardsiella tarda, for Use As a Disease Biocontrol Agent2018: National Research Council of Thailand (NRCT); 286,000 Baht
Detection of a plasmid containing cadmium resistance gene for lactic acid bacteria isolated from foods
Publication
Books
1. ปาริชาติ พุ่มขจร. 2560. หลักพันธุวิศวกรรมและการประยุกต์ใช้ในงานวิจัย. สำนักพิมพ์แห่งจุฬาลงกรณ์มหาวิทยาลัย. กรุงเทพฯ.
2. ปาริชาติ พุ่มขจร. 2561. ภูมิคุ้มกันวิทยา. สำนักพิมพ์แห่งจุฬาลงกรณ์มหาวิทยาลัย. กรุงเทพฯ.
International Publications1. Rattanachaikunsopon P, Phumkhachorn P: Lactic acid bacteria isolated from Thai fermented foods and their antibacterial activity. ASEAN Journal on Science and Technology for Development 1999, 16(2): 19-29.
2. Rattanachaikunsopon P, Phumkhachorn P: A bacteriocin produced by Lactobacillus lactis subsp. lactis isolated from Thai fermented foods. ScienceAsia 2000; 26(4): 195-200.
3. Rattanachaikunsopon P, Phumkhachorn P: Isolation and preliminary characterization of a bacteriocin produced by Lactobacillus plantarumN014 isolated from nham, a traditional Thai fermented pork. Journal of Food Protection 2006, 69(8): 1937-1943.
4. Phumkhachorn P, Rattanachaikunsopon P, Khunsook S: The use of gfp gene in monitoring bacteriocin-producing Lactobacillus plantarum N014, a potential starter culture in nham fermentation. Journal of Food Protection 2007, 70(2): 419-424.
5. Rattanachaikunsopon P, Phumkhachorn P: Bacteriostatic effect of flavonoids isolated from leaves of Psidium guajava on fish pathogens. Fitoterapia 2007, 78(6): 434-436.
6. Rattanachaikunsopon P, Phumkhachorn P: Incidence of nisin Z production in Lactococcus lactis subsp. lactis TFF 221 isolated from Thai fermented foods. Journal of Food Protection 2008, 71(10): 2024-2026.
7. Rattanachaikunsopon P, Phumkhachorn P: Characterization of nisin produced by Lactococcus lactis RP359 isolated from Kem-Buk-Nud, a traditional Thai fermented food. The Internet Journal of Microbiology 2008, 5(1).
8. Rattanachaikunsopon P, Phumkhachorn P: Diallyl sulfides content and antimicrobial activity against foodborne pathogenic bacteria of chives (Allium schoenoprasum). Bioscience, Biotechnology and Biochemistry 2008, 72(11): 2987-2991.
9. Pachanawan A, Phumkhachorn P, Rattanachaikunsopon P: Potential of Psidium guajava supplemented fish diets in controlling Aeromonas hydrophila infection in tilapia(Oreochromis niloticus). Journal of Biotechnology and Bioengineering 2008, 106(5): 419-422.
10. Rattanachaikunsopon P, Phumkhachorn P: Prophylactic effect of Andrographis paniculata extracts against Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus). Journal of Biotechnology and Bioengineering 2009, 107(5): 579-582.
11. Rattanachaikunsopon P, Phumkhachorn P: Antimicrobial activity of elephant garlic oil against Vibrio cholerae both in vitro and in food model. Bioscience, Biotechnology and Biochemistry 2009, 73(7): 623-627.
12. Rattanachaikunsopon P, Phumkhachorn P: Glass bead transformation method for Gram-positive bacteria. Brazilian Journal of Microbiology 2009, 40: 923-926.
13. Rattanachaikunsopon P, Phumkhachorn P: Protective effects of clove oil supplemented fish diets on experimental Lactococcus garvieae infection in tilapia. Bioscience, Biotechnology and Biochemistry 2009, 73(9): 2085-2089.
14. Rattanachaikunsopon P, Phumkhachorn P: Glass bead-based transformation method for lactic acid bacteria. ScienceAsia 2009, 35:234-241.
15. Rattanachaikunsopon P, Phumkhachorn P: Potential of Chinese chive oil as a natural antimicrobial for controlling Flavobacterium columnare infection in Nile tilapia Oreochromis niloticus. Fisheries Science 2009, 75:1431-1437.
16. Rattanachaikunsopon P, Phumkhachorn P: Shallot (Allium ascalonicum L.) oil: diallyl sulfide content and antimicrobial activity against food-borne pathogenic bacteria. African Journal of Microbiology Research 2009, 3:747-750.
17. Rattanachaikunsopon P, Phumkhachorn P: In vitro study of synergistic antimicrobial effect of carvacrol and cymene on drug resistant Salmonella typhi. African Journal of Microbiology Research 2009, 3:978-980.
18. Rattanachaikunsopon P, Phumkhachorn P: Use of Centella asiatica aqueous extract as bath treatment to control columnaris in tilapia (Oreochromis niloticus). Journal of Aquatic Animal Health 2010, 22:14-20.
19. Rattanachaikunsopon P, Phumkhachorn P: Potential of coriander (Coriandrum sativum) oil as a natural antimicrobial in inhibiting Campylobacter jejuni in raw meat. Bioscience, Biotechnology and Biochemistry 2010, 74:31-35.
20. Rattanachaikunsopon P, Phumkhachorn P: Synergistic antimicrobial effect of nisin and ρ-cymene against Salmonella enterica serovar Typhi in vitro and on ready-to-eat food. Bioscience, Biotechnology and Biochemistry 2010, 74:520-524.
21. Rattanachaikunsopon P, Phumkhachorn P: Potential of cinnamon (Cinnamomum verum) oil in controlling Streptococcus iniae infection in tilpia (Oreochromis niloticus). Fisheries Science 2010, 76:287-293.
22. Rattanachaikunsopon P, Phumkhachorn P: Contents and antibacterial activity of flavonoids extracted from leaves of Psidium guajava. Journal of Medicinal Plants Research 2010, 4:393-396.
23. Rattanachaikunsopon P, Phumkhachorn P: Assessment of synergistic efficacy of carvacrol and cymene against Edwardsiella tarda in vitro and in tilapia (Oreochromis niloticus). African Journal of Microbiology Research 2010, 4:420-425.
24. Thamniamton W, Boonsarn V, Phumkhachorn P, Rattanachaikunsopon P: Isolation and characterization of a bacteriophage specific to drug resistant Klebsiella pneumoniae DR1. International Journal of Current Research and Review 2010, 2 (4):30-43.
25. Rattanachaikunsopon P, Phumkhachorn P: Effect of Cratoxylum formosum on innate immune response and disease resistance against Streptococcus agalactiae in tilapia Oreochromis niloticus. Fisheries Science 2010, 76:653-659.
26. Rattanachaikunsopon P, Phumkhachorn P: Antimicrobial activity of basil (Ocimum basilicum) oil against Salmonella Enteritidis in vitro and in food. Bioscience, Biotechnology and Biochemistry 2010, 74:1200-1204.
27. Phumkhachorn P, Rattanachaikunsopon P: Isolation and partial characterization of a bacteriophage infecting the shrimp pathogen Vibrio harveyi. African Journal of Microbiology Research 2010, 4:1794-1800.
28. Rattanachaikunsopon P, Phumkhachorn P: Assessment of factors influencing antimicrobial activity of carvacrol and cymene against Vibrio cholerae in food. Journal of Bioscience and Bioengineering 2010, 110:614-619.
29. Rattanachaikunsopon P, Phumkhachorn P: Lactic acid bacteria: their antimicrobial compounds and their uses in food production. Annals of Biological Research 2010, 1:218-228.
30. Rattanachaikunsopon P, Phumkhachorn P: Bacteriophages: Discovery and therapeutic uses in humans and animals. International Journal of Current Research and Review 2010, 2 (11):3-8.
31. Phumkhachorn P, Rattanachaikunsopon P: Bacteriophage specific to nisin producing-Lactococcus lactissubsp. Lactis TFF221, a starter culture in Thai fermented food. African Journal of Microbiology Research 2011, 5(6): 1203-1210.
32. Rattanachaikunsopon P, Phumkhachorn P: Detection of Aeromonas salmonicida by reverse transcription-multiplex polymerase chain reaction. Bioscience, Biotechnology and Biochemistry 2012, 76(4):665-670.
33. Rattanachaikunsopon P, Phumkhachorn P: Identification of viable Listeria species based on reverse transcription-multiplex PCR (RT-PCR) and restriction digestion. Bioscience, Biotechnology and Biochemistry 2012, 76(6): 1189-1194.
34. Rattanachaikunsopon P, Phumkhachorn P: Construction of food-grad cloning vector for Lactobacillus plantarum and its utilization in food model. Journal of General and Applied Microbiology 2012, 58(4): 317-324.
35. Phumkhachorn P, Rattanachaikunsopon P: Isolation and characterization of lytic phage against Lactococcus lactisRP359, kem-buk-nud starter culture. African Journal of Microbiology Research 2012, 6(37): 6678-6684.
36. Butprom S, Phumkhachorn P, Rattanachaikunsopon P: Effect of Lactobacillus plantarum C014 on innate immune response and disease resistance against Aeromonas hydrophila in hybrid catfish. The Scientific World Journal 2013, 2013:1-6.
37. Rattanachaikunsopon P, Phumkhachorn P: Bacteriophage ϕLPN014 infecting Lactobacillus plantarum N014, a potential starter culture for NHAM fermentation. Annals of Experimental Biology 2014, 2(1):1-7.
38. Somnate T, Phumkhachorn P, Rattanachaikunsopon P: Potentail of virulent bacteriophage as a biocontrol agent against Salmonella Typhimurium in beverages. Journal of Pure and Applied Microbiology 2014, 8(2): 1131-1139.
39. Phumkhachorn P, Rattanachaikunsopon P: A lytic bacteriophage with potential for inactivation of a fish pathogenic Streptococcus agalactiae. Journal of Pure and Applied Microbiology 2014, 8(Spl. Edn. 2):371-379.
40. Punyauppa-path S, Phumkhachorn P, Rattanachaikunsopon P: Nisin: Production and mechanism of action. International Journal of Current Research and Reviews 2015, 7(2): 47-53.
41. Phumkhachorn P, Rattanachaikunsopon P: Therapeutic use of Cassia alata aqueous extract as bath treatment to control Pseudomonas anguilliseptica infection in tilapia (Oreochromis niloticus). Archives of Biological Sciences 2015, 67(4): 1165-1172.
42. Punyauppa-path S, Phumkhachorn P, Rattanachaikunsopon P: Factors influencing synergistic antimicrobial activity of thymol and nisin against Shigella spp. in sugarcane juice. Biologia 2015, 70(8): 1003-1010.
43. Phumkhachorn P, Rattanachaikunsopon P: A Siphoviridae bacteriophage specific to Extended-spectrum b-lactamases-producing Escherichia coli. Journal of Chemical and Pharmaceutical Research 2015, 7(11): 604-608.
44. Phumkhachorn P, Rattanachaikunsopon P: A broad host range food-grade cloning vector for lactic acid bacteria. Biologia 2016, 71(5):457-463.
45. Phumkhachorn P, Rattanachaikunsopon P: Detection of viable Salmonella Typhi using three primer pairs specific to invA, ivaB and fliC-d genes. Emirates Journal of Food and Agriculture 2017, 29(4): 312-316.
46. Rattanachaikunsopon P, Phumkhachorn P. Bacteriophages as Future Prophylactic and Therapeutic Agents against Foodborne Bacterial Pathogens. International Journal of Nutrition and Health Sciences 2017, 2(1): 19-20.
47. Rattanaborvorn W., Phumkhachorn P, Rattanachaikunsopon P: Potential of Bacteriophages in Controlling Drug Resistant Shigella sonnei. Asian Journal of Microbiology, Biotechnology and Environmental Science 2017, 19(3): 526-530.
48. Phumkhachorn P, Rattanachaikunsopon P: Bacteriophage therapy as a future preventive and curative approach against bacterial infections in aquaculture. Multidisciplinary Advances in Veterinary Science 2017, 1(4): 167-168.
49. Phumkhachorn P, Rattanachaikunsopon P: Potential of bacteriophage PUB 36 to enhance nonspecific immune response and disease resistance of tilapia (Oreochromis niloticus). Asian Journal of Microbiology, Biotechnology and Environmental Sciences 2018, 20(Feb. Suppl.): S69-S73.
50. Phumkhachorn P, Rattanachaikunsopon P: Multiplex PCR based detection of Salmonella enterica serovars Typhi, Paratyphi A, B and C in drinking water and food. Asian Journal of Microbiology, Biotechnology and Environmental Sciences 2018, 20(2): 493-500.
51. Phumkhachorn P, Rattanachaikunsopon P: A lytic podoophage specific to fish pathogenic Edwardsiella tarda. Pakistan Journal of Biotechnology 2018, 15(1): 117-121.
52. Rattanachaikunsopon P, Phumkhachorn P: Functional food: what are they? and why are they so popular? Acta Scientific Nutritional Health 2018, 2(11): 26-27.
53. Rattanachaikunsopon P, Phumkhachorn P: Detection of a plasmid containing cadmium resistance gene for lactic acid bacteria isolated from foods. Asian Journal of Agriculture and Biology 2018, 6(4): 530-534.
54. Pornnikom P, Phumkhachorn P, Rattanachaikunsopon P: Synergistic Effect of Bacteriophage and Ampicillin against Shigella dysenteriae. Bio Bulletin 2019, 5(1): 5-9.
55. Phumkhachorn P, Kamsa S, Rattanachaikunsopon P: Effect of temperature on antimicrobial activity and mode of action of thymol against Staphylococcus aureus. Indian Journal of Science and Technology 2019, 12(15): 1-5.
56. Phumkhachorn P, Rattanachaikunsopon P: Chlorpyrifos degrading Pseudomonas stutzeri isolated from pesticide contaminated soil. Asian Journal of Agriculture and Biology 2020, 8(3): 268-273.
57. Phumkhachorn P, Rattanachaikunsopon P: Use of bacteriophage to control experimental Aeromonas hydrophila infection in tilapia (Oreochromis niloticus). Pakistan Journal of Biological Sciences 2020, 23(12): 1659-1665.
58. Rattanachaikunsopon P, Phumkhachorn P: A glimpse of COVID-19 situation in Thailand. International Journal of Current Research and Review 2020, 12 (23): 1-2.
59. Rattanachaikunsopon P, Phumkhachorn P: New Surge of COVID-19 Infected Cases in Thailand. International Journal of Current Research and Review 2021, 13 (2): 1.
60. Rattanachaikunsopon P, Phumkhachorn P: Third wave of COVID-19 in Thailand. International Journal of Current Research and Review 2021, 13 (9): 1-2.
61. Rattanachaikunsopon P, Phumkhachorn P: Antimicrobial synergism between bacteriophage UBU-SA1 and oxacillin against Staphylococcus aureus. International Journal of Current Research and Review 2021, 13 (19): 173-177.
62. Rattanachaikunsopon P, Phumkhachorn P: Status of COVID-19 vaccines in Thailand. Scientist. 2022, 1(1): 26-30.
63. Phumkhachorn P, Rattanachaikunsopon P: Fighting approaches against COVID-19: For now and the future. Bioscience Biotechnology Research Communications. 2022, 15(2)-269-271.
64. Phumkhachorn P, Rattanachaikunsopon P: Teixobactin: A powerful antibiotic from natural resource. Ecology, Environmental and Conservation. 2022, 28(3): 1103-1107.
65. Phumkhachorn P, Rattanachaikunsopon P: Lactobacillus gasseri with probiotic properties isolated from Thai traditional fermented foods. Annals of Agri Bio Research. 27(2): 211-216.
66. Punyauppa-path S, Kiatprasert P, Sawangkaew J, Mahakhan P, Phumkhachorn P, Rattanachaikunsopon P, Khunnamwong P, Srisuk N. Diversity of fermentative yeasts with probiotic potential isolated from Thai fermented food products. AIMS Microbiology. 2022, 8(4): 575-594.
67. Phumkhachorn P, Rattanachaikunsopon P: Probiotics: Sources, selection and health benefits. Research Journal of Biotechnology. 2023, 18(5): 102-113.
68. Phupaboon S, Hashim FJ, Phumkhachorn P, Rattanachaikunsopon P. Molecular and biotechnological characteristics of proteolytic activity from Streptococcus thermophilus as a proteolytic lactic acid bacteria to enhance protein-derived bioactive peptides. AIMS Microbiology. 2023, 9(4): 591-611.
69. Phumkhachorn P, Rattanachaikunsopon P: Effects of Azadirachta indica leaf aqueous extract supplemented fish diets on innate immune response and disease resistance of tilapia (Oreochromis niloticus). Research Journal of Biotechnology. 2024, 19(5): 1-8.